CD Skripsi
Isolasi Dan Karakterisasi Morfologi Dan Fisiologi Bakteri Endofit Dari Tanaman Buah Naga Merah (Hylocereus Polyrhizus)
Endophytic bacteria are bacteria that live in plant tissue, including in dragon
fruit plant tissue. There are many species of endophytic bacteria living inside
tissue dragon fruit plant wich play a beneficial role for plant. Endophytic bacteria
can be used as a biological fungicide and biological fertilizer which are useful for
plants. The purpose of the research is to observe the characteristics of the
endophytic bacteria. Plants samples was taken by using purposive sampling
method. The location is at Balai Pelatihan dan Pengembangan Masyarakat PT.
Arara Abadi the district of Siak Riau. Plant tissues were taken from roots and
shoots of the dragon fruit plants. The data were analyzed descriptively. Results
showed that the morphological characterization of endophytic bacteria such as
colonies color, colonies form, and colonies elevation had some similarities each
others, but there was a difference on the edges of colony morphology. Two
isolates had a flat edges and three isolates have wavy edges. Physiologycal assays
such as catalase assay, oxidase assay and starch hydrolysis assay of the bacteria
showed a positive on the each isolate, but there was a difference on the motility
assay and indole assay. Two isolates showed that they were motile and positive
indole assay, while the three other isolates showed that they were non-motile and
indol negative assay. Based on the morphological and physiological similarities of
the endophytic bacteria, it can be assumed the bacteria belong to Genera of
Bacillus and Corynebacterium.
Keywords : Isolation, characterization, endophytic bacteria, and dragon fruit
plant
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