CD Skripsi

Produksi Enzim Lakase Oleh Jamur Trichoderma Asperellum Lbkurcc1 Dalam Bioreactor Tray Dengan Memvariasikan Ukuran Substrat Jerami Padi Dan Induser Cuso4 Secara Fermentasi Kultur Padat

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Lakase is an enzyme that can oxidize phenolic compounds and potentially to be utilized in various industrial fields such as lignin degradation in pulp, degradation of toxic pollutants in bioremediation, as microbes in the process of fermentation of vegetable raw materials to produce bioethanol and color degradation in textile industry waste. In this research, the production of lacase enzyme using Trichoderma asperellum LBKURCC1 with bioreactor tray using solid state fermentation (SSF) method with rice straw substrate. The purpose of this study was to determine the effect of rice straw size and the addition of CuSO4 concentration to the highest production of lacase enzyme by Trichoderma asperellum LBKURCC1. Fermentation is carried out with variation of time 5, 6, 7, 8, 9 and 10 days with fermentation temperature ± 30ºC, substrate size ± 0.5 cm, 1.5 cm, and 3.0 cm with substrate thickness at tray ie 3 cm acetate buffer solution pH 5,5 and addition of CuSO4 0.5 g/l. Variations for the addition of CuSO4 concentration 0 g/l, 0.50 g/l and 1 g/l with substrate thickness on tray were 3 cm, size of rice straw 0.5 cm and acetate buffer solution pH 5.5. Small size can provide the highest value and the added concentration of CuSO4 given can increase the activity of the resulting lacase enzyme. However, too high CuSO4 concentrations result in decreased lacase enzyme activity. The results showed that the highest lacase enzyme activity was obtained on the size of rice straw ie 0.5 cm and 7 days of fermentation time with an average of 19.27 U/L and the highest lacase enzyme activity was obtained on CuSO4 0.50 g/l and fermentation time 7 day with an average of 19.27 U/L.
Keywords: Bioreactor tray, Rice straw, Laccase, Trichoderma asperellum, Solid state fermentation

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

07 02. 118 (0060)

Penerbit

Pekanbaru : Universitas Riau – Fakultas Teknik – Teknik Kimia., 2018

Deskripsi Fisik

xii, 40 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

07 02. 118 (0060)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

Versi lain/terkait

Lampiran Berkas

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