CD Disertasi

KEANEKARAGAMAN BAKTERI HIDROKARBONOKLASTIK DI AREA EKSPLORASI PETAPAHAN DAN KEMAMPUANNYA DALAM MENDEGRADASI MINYAK BUMI

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The management of natural oil resources has various impacts on human life and the environment. These impacts can be positive and negative. The negative impacts caused include waste (residue) which is very dangerous to the environment causing damage to the ecosystem in this area. This study aims to: 1) Identify the diversity of biomolecular hydrocarbonoclastic bacteria in the exploration area of PT. Chevron in Riau Province. 2) To determine the ability of hydrocarbonoclastic bacteria to degrade petroleum and the growth profile of hydrocarbonoclastic bacteria. 3) To determine the ability of mixed cultures to degrade petroleum. 4) To determine the optimum conditions of hydrocarbonoclastic bacteria in degrading petroleum. 5) To implement the bioremediation process using the best optimization results on a laboratory scale with a simple bioreactor. This research was carried out descriptively with experiments in the laboratory. This study consisted of 5 (five) stages: Phase I. Diversity of biomolecular hydrocarbonoclastic bacteria in the exploration area of PT Cevron Pasifik Indonesia Riau. Stage II. For a single culture and manufacture of hydrocarbonoclastic bacterial profiles. Stage III. Analyze the culture test of a mixture of hydrocarbonoclastic bacteria in degrading petroleum. Stage IV. To determine the optimum conditions for petroleum biodegadation processes, petroleum concentration optimization, optimization of inoculum concentration, optimization of agitation optimization pH and temperature optimization. Stage V. Implementation The biodegradation process uses the best optimization results in a simple bioreactor. Analyzing the relationship of bacterial isolates Petroleum, growth time, pH level, temperature, concentration of inoculum. Concentration of petroleum to petroleum degradation at TPH and COD levels. Stage VI. bacterial isolates Petroleum with Partial Least Square (PLS) modeling, growth time, pH, temperature, inoculum concentration. the concentration of petroleum to petroleum degradation at TPH and COD levels. Stage VII. estimate the environmental cost efficiency resulting from the implementation of hydrocarbonoclastic bacterial isolates. The results of the study obtained seven petroleum isolates, namely: 1) Phylobacterium myrsynacearum strain IMB-05,2) Gardonia terrae strain IMB-07, 3) Pseudomonas tollasii strain IMB-09, 4) Bacillus cereus strain IMB-10, 5) Bacillus toyonensis strains of IMB-11, 6) Lynisinibacillus fusiformis strain 1MB-12, and 7) Pseudomonas stutzeri Strain IMB-15. The best test results of mixed culture in degrading petroleum are consortium of 7 isolates with TPH degradation rate of 77.70% and COD 89.83 %. The best optimization results obtained optimum consortium growth with 20% petroleum concentration, 15% inoculum concentration, pH 7.5, 34oC temperature and 130 rpm agitation speed with TPH degradation rate 93.50% and COD 93.39%. Based on the results of the economic value analysis of petroleum bioremediation process using biological agents (bacteria) it is estimated that the cost required for 1 barrel is $ 12,676.
Keywords: Petroleum, Hydrocarbonoclastic Bacteria, Total Hydrocarbon Petroleum (TPH), Chemical Oxygen Demand (COD), Degradation.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 01. 318 (0003)

Penerbit

Pekanbaru : Universitas Riau – Pasca Sarjana – Disertasi Ilmu Lingkungan., 2018

Deskripsi Fisik

xxii, 155 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 01. 318 (0003)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (DISERTASI) ILMU LINGKUNGAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

DODY

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