CD Tesis

Efektivitas Bakteri Simbiotik Penambat Nitrogen (Rhizobium) Asal Tanah Gambut Riau Dalam Memacu Pertumbuhan Acacia Crassicarpa A. Cunn. Ex Benth Di Pembibitan

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The aim of this study is to obtain suitable, effective and efficiento of isolate Rhizobium bacteria in increasing A. crassicarpa growth in nursery and to know the species of symbiotic nitrogen-fixing bacteria that associated with A. crassicarpa plant. The symbiotic nitrogen-fixing bacteria isolated from root nodules of A. crassicarpa that collected from several trees were planted in several peat land in Riau. Bacteria from isolation results, performed authentication test to determine its ability to form root nodule on A. crassicarpa plant. five best isolates from the authentication test results continued in the nursery test. Research in the nursery using split-split plot design as main plot is sterilization of planting media consist of two level that is: S1: sterile media and S2: media without sterilization. Subplot factor is fertilizer application consisting of 3 levels: P1: 100% P and K fertilizer without Nitrogen, P2: N, P and K fertilizer dose 50% and P3: 100% of N, P and K fertilizer. Sub-sub plot factor is application of isolate Rhizobium bacteria consisting of 6 level that is: control without inoculation of Rhizobium bacteria isolates and 5 Rhizobium bacteria isolates. Variable observed included: Survival, plant height, diameter of root collar (DRC), leaf number, leaf area, fresh weight, plant biomass, amount, weight and volume of nodules, nitrogen nutrient uptake value. Identification of selected symbiotic nitrogen bacterial isolates from nursery test was identified using the analysis technology of 16S rRNA gene sequences.
Exploration and isolation result were found as many as 54 isolates of Rhizobium bacteria, and got 5 isolates of selected Rhizobium bacteria from the authentication test of 54 Rhizobium bacteria isolates. And the five isolates were: isolates BBRD01, RSKA14, RSKA05, RSKA09 and PSKA04. The experimental results in the nursery indicated that Sterilization of planting medium could increase plant survival, but did not increase the height growth, DRC, leaf number, leaf area, fresh weight, biomass, nodule amount, nodule weight, nodule volume, and nitrogen uptake of A. crassicarpa plant in the nursery. Fertilizer application can increase plant survival, high growth, DRC, leaf number, leaf area, fresh weight, biomass, nodule amount, nodule weight, nodule volume, and nitrogen uptake of A. crassicarpa plant in nursery. Inoculation of Rhizobium bacterial isolates can increase survival rate and high growth, DRC, leaf number, leaf area, fresh weight, biomass, nodule amount, nodule weight, nodule volume, and nitrogen uptake of A. crassicarpa plant in nursery. Rhizobium bacterial isolates BBRD01 and RSKA14 are the most effective isolates in increasing plant height growth, DRC, leaf number, leaf area, fresh weight, biomass, nodule amount, nodule weight, nodule volume, and nitrogen uptake of A. crassicarpa plant in the nursery. Nucleotide sequence result of selected Rhizobium bacteria isolates (BBRD01, RSKA05 and RSKA14) had the closest relatives with Rhizobium tropici with Max identity of 97%, 94% and 93% respectively. The phylogenetic tree showed that Rhizobium bacteria isolates obtained at the same site (RSKA05 with RSKA14) had the closest kinship levels, and vice versa (RSKA05 and RSKA14 with BBRD01).

Key word: Rhizobium bacteria, Nitrogen, Peat, Acacia crassicarpa.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 10. 218 (0004)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Pertanian., 2018

Deskripsi Fisik

xxii, 169 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 10. 218 (0004)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) ILMU PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

Lampiran Berkas

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