CD Tesis

Isolasi Dan Uji Potensi Bakteri Fiksasi N Non Simbiotik Dan Bakteri Nitrifikasi Asal Tanah Kebun Kelapa Sawit Dengan Aplikasi Tandan Kosong Dan Limbah Cair Kelapa Sawit

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This study aimed was to isolate non symbiotic N fixation bacteria from oil palm plantations fertilized with empty fruit bunch (EFB) and palm oil mill effluent (POME) and to analyze their potency. The soil samples were sampled from private oil palm plantations in Pelalawan, Riau. The soil samples were taken by purposive sampling method from 4 different treatments ie mixed application (EFB + POME), EFB application, POME application and without application(C), and each location was taken 3 times. Isolation of non symbiotic N fixation bacteria was perfomed by using NFb and Ashby's media. Isolation of nitrifiying bacteria was perfomed by using Nitrosomonas sp. and Nitrobacter sp. spesific bacteria. The parameters observed were bacterial cells number, number of isolates and their potency.
The results showed cells number of non symbiotic N fixing bacteria ranges 3.62 – 9.45 x 105 cfu/g soil. The highest total bacteria population was obtained at mixed treated area (EFB+POME), more POME application, EFB application and the lowest population was obtained at without application (C). A total of 18 isolates were isolated and potentially as a nitrogen fixing bacteria characterized by the ability of isolates to change the color of the NFb medium from green to blue and form pellicle. The highest potential isolates are NFBC232, NFBK21, and NFBC33. Characteristics of colony morphology were found to vary and morphology of round cells and stems, there were 2 Gram positive isolates and 16 Gram negative isolates.
The cells number of nitrifying bacteria at the location of palm oil and without applications, the population is not much different from 8.0 - 11.40 x 105 cfu g-1 soil. A total of 18 isolates were isolated and potentially oxidized ammonium and nitrate.The best potential of nitrifying bacteria in oxidizing ammonium at a concentration of 500 ppm (NH4)2SO4 was isolated NSC34 (3.4 ppm) derived from soil samples of mixed application locations. Isolates with the best potential to produce nitrate at a concentration of 500 ppm (NH4)2SO4 were isolates of NBC225 (26.1 ppm) isolates derived from soil samples of mixed application locations. The most optimal activity of isolate bacteria to nitrifiying occurred at a concentration of 500 ppm (NH4)2SO4 and at 4 days incubation time. The morphological characteristics of colonies found varied and cell morphology was spherical, stem and spiral and all isolates including Gram negative.

Keywords: Non symbiotic N fixing bacteria, Nitrifiying bacteria, Empty fruit bunch, Palm oil mill effluent

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 10. 218 (0006)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Pertanian., 2018

Deskripsi Fisik

xvi, 78 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 10. 218 (0006)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) ILMU PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

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