CD Tesis

Karakteristik Dan Efektivitas Kitosan Karapas Udang Rama-Rama (Thalassina Anomala) Sebagai Senyawa Antibakteri

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T. anomala is a type of shrimp that commonly found in the muddy areas of mangrove along the coast of Selat Panjang. This study aims to investigate the clear zones of the chitosan obtained from that shrimp that is tested against Pseudomonas aeruginosa. Carapace from T. anomala is used as chitosan’s raw material resources. Analysis was carried out at the Integrated Laboratory, Fisheries Microbiology Laboratory, Fisheries Product Technology Department, Faculty of Fisheries and Marine Sciences, University of Riau; Material Analysis Laboratory, Department of Physics, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. A CRD (99% confidence limit) with 5 treatments, namely positive control (K0+), negative control (K0-), 5% chitosan (K1), 7% chitosan (K2) and 9% (K3) was applied and then was continued with a Duncan test. This research consists of 3 main procedures, namely: 1) preparation and chemical analysis of carapace, 2) extraction and chemical analysis of chitin and chitosan, 3) chitosan extraction and antibiotic test against the bacteria. Parameters measured were the shrimp rendement value, proximate test (moisture content, ash, protein, fat and carbohydrate), mineral analysis (Ca, Fe, K, Na, P), deacetylization degree and clear zones.
The proximate carapace test results were as follows,water content was 68.18 % gross weight (gw), moisture content 11.77 % dry weight (dw), protein content 2.35 %dw, fat content 84,43 %dw and carbohydrate content was 1,45 %dw. The chitosan of T. anomala characteristics were yellowish white flakes, odorless and tasteless with yield value, moisture content, ash content, mineral content (Ca, Fe, K, Na, P) and the degree of deacetylation 27.51%; 7.31 %gw; 3.41 %dw; 12.17 mg/L; 4.90 mg/L; 3.40 mg/L; 5.49 mg/L; 0.27 mg/L; and 73.43% respectively, showed that chitosan T. anomala had met the quality standard but the ash content was relatively high so it still did not meet the standard results on antimicrobial sensivity tests using double layer, disk blank and well diffusion. Shown clear zone, 13.87 mm, 11.34 mm and 14.62 mm. these values indicate that the antimicrobial effect of the chitosan is strong.


Key words : T. anomala, chitosan, clear zone

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 16. 219 (0001)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Kelautan., 2019

Deskripsi Fisik

x, 92 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 16. 219 (0001)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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