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Identifikasi Bakteri Penghasil Antibiotik Dari Mikrohabitat Di Ekosistem Mangrove Secara Molekuler Dan Aktivitasnya Terhadap Bakteri Patogen

The presence of antibiotic-producing bacteria from microhabitat in mangrove ecosystems can be useful to produce new antibiotics for industrial and environmental conservation. Mangrove ecosystems in Dumai and Siak have the potential to possess antibiotic-producing bacteria that have not been much studied. The objective of this research was to obtain antibiotic-producing bacteria from extreme microhabitats in the mangrove ecosystem in suppressing the growth of pathogenic bacterial pathogens (Vibrio alginolyticus, Aeromonas hydrophila, Edwardsiella tarda, Pseudomonas sp, Edwardsiella ictaluri dan Escherichia coli Samples were taken from several microhabitat mangrove areas in Dumai (Kelurahan Purnama Dumai) and Siak (Desa Kayu Ara), Riau Province. The survey method is used for identification, antibacterial test against pathogenic bacteria (V. alginolyticus, A. hydrophila, E. tarda, Pseudomonas sp, E. ictaluri dan E. coli). Bacterial isolation was carried out using the pour plate method and purification was carried out using the quadrant streak method. Identification is carried out through observations of macroscopic, microscopic morphology, biochemical tests, antagonism tests (agar diffusion method on agar nutrient media) and DNA analysis. Sequencing of 16S rDNA from the best antibiotic-producing bacterial isolates indicated that molecularly identified of Bacillus amyloliquefaciens, Bacillus cereus, Enterobacter hormaechei, Klebsiella pneumonia, and Enterococcus gallinarum. All bacteria were able to inhibit the growth of V. alginolyticus, A. hydrophila, E. tarda, Pseudomonas sp, E. ictaluri dan E. coli.

Keywords: Antibiotic Producing Bacteria, Pathogenic Bacteria, Identification, and Extreme Microhabitat

Ketersediaan
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Perpustakaan Universitas Riau 10 16. 219 (0016)
10 16. 219 (0016)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
10 16. 219 (0016)
Penerbit
Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Kelautan.,
Deskripsi Fisik
xiii, 116 hlm.; ill.; 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
10 16. 219 (0016)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
PASCASARJANA (MAGISTER) ILMU KELAUTAN
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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