CD Tesis

Kemampuan Biodegradasi Bakteri Selulolitik Pada Ekosistem Mangrove

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Utilization of biological compounds such as enzymes derived from microorganisms such as bacteria and fungi is currently increasing, in line with their development and necessary in various industries. In the field of fisheries, cellulase enzymes are also known to be used in treating organic waste in shrimp ponds cultivation and waste from seaweed processing. Enzyme-producing microorganisms are often found in nature, such as in water, soil and biota, including mangrove ecosystems. Litter derived from mangrove leaves that fall to the ground is a place to live for bacteria, fungi and other microorganisms. In addition litter is the main source of cellulose, thus allowing cellulose content in mangrove soils is high. Extracellular enzymes (proteolytic, amylolytic, lipolytic and cellulase) are enzymes produced by bacteria found in sediments from mangrove ecosystems. The process of cellulose decomposition is very dependent on the presence of cellulase enzymes possessed by decomposing microorganisms, namely cellulolytic bacteria. This decomposition of bacterial activity causes high productivity of the mangrove ecosystem. Dumai Marine Station in Riau Province has a fairly extensive mangrove forest conservation area, and has various types of mangroves. Many studies have been carried out on this mangrove ecosystem, including on macrobiota, nutrients and heavy metal analysis. But microbiological research regarding cellulose degrading microorganisms is still not much done. Given the importance of the presence of bacteria, especially cellulolytic bacteria as nutrients recyclers in the mangrove ecosystem, and their use in the field of biotechnology, it is necessary to conduct microbiological research on the mangrove ecosystem at the Dumai Marine Station. The purpose of this study is to obtain cellulolytic myrobes, find out the optimum time of cellulolytic enzyme production and how the degradation ability of selected cellulolytic bacteria to mangrove litter. The results showed that from isolation, selection (identification of microbes in mangrove forest sediments at the Dumai Marine Station), 24 isolates were obtained that had the ability to grow in media containing CMC with the highest cellulolitic activity found in isolates BS.ST2.8. The results of the phenotype and genotype isolates of cellulolytic bacteria were known that the three isolates were B. toyonensis. Most cellulase enzymes produced by bacterial isolate BS.ST2.8 occurred at the 78th hour after incubation. Based on the results of further tests using several doses of cellulase enzyme isolates BS.ST2.8 against mangrove litter substrate, it is known that generally can degrade coarse fibers in mangrove litter at a dose of 50% decrease in NDF, ADF, cellulose and hemicellulose fiber fractions.
Keywords: biodegradation ability, cellulolytic bacteria.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 16. 219 (0027)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Kelautan., 2019

Deskripsi Fisik

xv, 96 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 16. 219 (0027)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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