CD Skripsi
Vaksinasi Ikan Lele Dumbo (Clarias Gariepinus) Dari Bakteri Aeromonas Hydrophila Yang Diisolasi Dari Ikan Lele Dumbo (Clarias Gariepinus) Dengan Dosis Yang Berbeda
ABSTRACT
A vaccine is an extracellular substance or antigen that has lost its pantogenicity by being weakened or turned off to increase the specific defenses of fish against a particular disease. The use vaccine was done by isolating the bacteria Aeromonas hydrophila from Clarias gariepinus that were conventionally identified. The technique of the use vaccine was done by adding 3% of formalin solution and then the viability were tested. The purpose of this research was to obtained the effect of A. hydrophila vaccine on survival rate (SR), relative protection level (RPS) and to the best A. hydrophila vaccine that were immersed to prevent Motile Aeromonas Septicemia (MAS) disease. The challenge test was carried out on the 23rd day will A. hyrophila with a density of 107 cell/ml. The fishes were infected intramuscularly. The method used in this study was an experimental method using a Completely Randomized Design (CRD) of a factor of five levels of treatment, three replications to obtain 15 experimental units. The treatments were applied namely Kn (negative control), Kp (positive control), the fishes were given A. hydrophila vaccine of P1: (0.5 ml), P2: (1 ml) and P3: (1.5 ml). After that, the fishes were soaked with A. hydrophila vaccine on the first day and the seventh day (booster) for 15 minutes. The results shown that the A. hydrophila vaccine that were isolated from Clarias gariepinus is able to prevent the Motile Aeromonas Septicemia (MAS) disease. The best done was in P2 with a survival rate (SR) of 63.33% (P
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