CD Skripsi

Uji In Vitro Hidroksiapatit Berpori Yang Dilapisi Kitosan Menggunakan Larutan Sbf (Simulated Body Fluid)

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ABSTRACT

One of biological method for hydroxyapatite test is in vitro test, which is testing the material outside the system or not directly on living things. Some ways include using cell culture media, test tubes or SBF (Simulated Body Fluid) solutions. This research aims to hold in vitro test of porous ceramics coated with chitosan and determine the effect of variations of chitosan and HCO3- ions on the results of in-vitro test of porous ceramics coated with chitosan. This research was carried out by synthesis CaO from cockle shells followed by synthesis HA from a mixture of CaO and NH4H2PO4 using low-temperature hydrothermal method. Porous HA is made by mixing HA, potato starch and distilled water using the starch consolidation method. A coating solution is prepared by mixing 0.75%w/v and 1.50%w/v into 2%v/v 100 ml acetic acid. The porous HA is then dipped into the solution for 60 minutes then dried at room temperature for 24 hours. The sample was then immersed in a SBF (Simulated Body Fluid) solution with a variation of HCO3- ions 4.2 mM and 27 mM for 28 days. Samples were then analyzed using AAS, SEM-EDX, XRD and FTIR analyzes. The Ca2+ ion concentrations obtained were 85.9415-(-13.459) ppm with degradation rate of 4.20-0.10%. Samples have degree of crystallinity of 86.581%-88.067% with crystal diameter of 41.220-46.244 nm. The Ca/P value obtained were 2.791-2.821. All samples contain OH- and PO43- groups at 3572,32cm-1 and 1027,14 cm-1 which are HA constituent groups and there were chitosan constituents in the form of N-H, C-H, C-O and C=O at 3095,88 cm-1, 2857,66 cm-1, 1055,11 cm-1, and 1653,07 cm-1. The use of more chitosan as coating reduced the concentration of Ca2+ ions, degradation rate, degree of crystallinity and crystal diameter. The increasing of HCO3- ion concentration increased Ca2+ ions concentration, degradation rate, degree of crystallinity and crystal diameter but decreased Ca/P ratio. Long immersion time reduced Ca2+ ions concentration and degradation rate.

Keywords: chitosan, hydroxyapatite, in vitro test, SBF (simulated body fluid)

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

07 02. 119 (0196)

Penerbit

Pekanbaru : Universitas Riau – Fakultas Teknik – Teknik Kimia., 2019

Deskripsi Fisik

xi, 71 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

07 02. 119 (0196)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

Versi lain/terkait

Lampiran Berkas

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