CD Skripsi

Produksi Metabolit Sekunder Penicillium Sp. Lbkurcc34 Berpotensi Antibiotik Menggunakan Elisitor Staphylococcus Aureus Dengan Variasi Konsentrasi Glukosa

---

Penicillium sp. LBKURCC34 is one of fungus that was isolated in peat soil of primary forest Cagar Biosfer Giam Siak Kecil Bukit Batu (GKSBB) Riau Province and has been collected by Biochemistry Laboratory, Riau University. This fungus is a potential source for the discovery of metabolic secondary compound with antimicrobial activity. This study was done to produc secondary metabollites of Penicillium sp. LBKURCC34 with added stimulation from Staphylococcus aureus. Glucose concentration in media used was varied from 5 g/L (A Extract), 10 g/L (B Extract), dan 15 g/L (C Extract). Production of secondary metabolic were used batch fermentation method with liquid media for 14 day and were extracted with ethyl acetate. The ethyl acetate extract were evaporated with vacum rotary evaporator, then analyzed with Liquid Chromatography-Mass Spectrometry (LC-MS) instrument. Antimicrobial assay that were used was disc diffusion method against pathogen bacteria such as Staphylococcus aureus, Bacillus subtilis, Staphylococcus ephidermidis, Eschericia coli, Vibrio parahaemolyticus, Vibrio alginolyticus and fungal pathogen Candida albicans with concentration 200 μg/disc. Amoxicillin (25 μg/disc) and Ketakonazol (30 μg/disc) were used as a positive control. Extract C, which weighed 52,8 mg, showed the most satisfactory results, based on this study. Extract C had the highest antibacterial activity against E. coli, shown by inhibition zone of 4,975±0,746 mm, which was 36,63% higher than positive control Amoxicillin (3,641±0,774) mm. Extract C also has inhibition zone of 4,975±0,746 mm, highest among other extracts, and also 131,27% higher than Ketakonazol (0,825±0,281) mm. The LC-MS analyzed that Extract A, Extract B, and Extract C contained a lot of polar compound with molecular weight 84,96 m/z up to 655,37 m/z. In conclusion, the fermentation method of Penicillium sp. LBKURCC34 and Staphylococcus aureus with glucose concentration 15 g/L is the best composition to produce potential antimicrobial extract.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

03 02. 120 (0022)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2020

Deskripsi Fisik

xii, 50 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

03 02. 120 (0022)

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar