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Analisis Keanekaragaman Genetik Kacang Hijau (Vigna Radiata L.) Asal Provinsi Riau Berdasarkan Penanda G-Ssr (Genomic-Simple Sequence Repeat)

Mungbean (Vigna radiata L.) is an important legume after soybean and peanut.
This study aime to analyze the genetic diversity of 10 mungbean genotypes from
Riau Province using g-SSR (Genomic-Simple Sequence Repeat) marker. The
DNA total was extracted from fresh leaves then amplified using 8 primer pairs of
g-SSR. The PCR products were migrated on 2,5% agarose gel in 1X TBE buffer.
The DNA bands were converted into binary data such as “0” if the band was
absent and “1” if the band was present. The binary data was then computed using
NTSYS-pc version 2.02i by UPGMA method. The results showed that the
similarity coefficient of 10 genotypes was 38%-100%. The highest of similarity
coefficient (100%) was in between Bengkalis II bulk A and Pelalawan bulk A,
Pelalawan bulk B and Rokan Hulu II bulk B, Rokan Hilir bulk C and Siak bulk A.
The lowest of similarity coefficient (38%) was in between Bengkalis I bulk B and
Indragiri Hilir bulk C. In conclusion, the genetic diversity of 10 mungbean
genotypes from Riau Province was approximately 36%.
Keywords: DNA bulk, genetic diversity, genotype, g-SSR, mungbean, Riau

Ketersediaan
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Perpustakaan Universitas Riau 03 04. 117 (0002)
03 04.117 (0002)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 04. 117 (0002)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Biologi.,
Deskripsi Fisik
xiii. 39. hlm.: ill: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 04. 117 (0002)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
BIOLOGI
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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