CD Tesis

Skrining Kemampuan Lima Galur Trichoderma Isolat Riau Sebagai Penghasil Lakase Secara Kualitatif

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Laccase is an enzyme that catalyzes the oxidation process of various types of organic and inorganic compounds such as phenolic compounds. Because of its abilitles laccase is an important enzyme for industry, In the food, textile and pharmaceutical industries. This study aims to screen the potential of laccase production from several Trichoderma isolates collected by the Biochemistry Laboratory, namely T. asperellum LBKURCC2, T. aureoviride LBKURCC22, and T. virens LBKURCC70/71/85. Screening for the ability to produce laccase by fungal isolates from various sources is important for the discovery of laccase with novel properties. A quick and simple method for screening laccase-producing fungi is to grow a plug of fungal mycelia in solid media supplemented with a laccase substrate (guaiacol or 2,2'-azino-di-3-ethylbenzothiazol-6-sulfonate, ABTS), with or without CuSO4 addition. The media for sterile PDA was cooled (up to 50ºC) and added with guaiacol or ABTS solution which had been filter sterilized using a 0.45 micron membrane filter. The agar medium was poured into a petri dish and allowed to solidify. A mycelial plug (1cm) was placed on the center of the media. The plates were incubated at room temperature (30ºC) and observed for up to 2 weeks. The presence of laccase activity is indicated as a brown halo circle for guaiacol and a dark green halo circle for ABTS around the fungi. The purpose of using CuSO4 is to increase laccase activity, because Cu2+ is an ion involved in the catalytic process of laccase. The results showed that laccase activity could be detected on day 3 and day 6 for T. asperellum LBKURCC2 and T. aureoviride LBKURCC22 on media containing guaiacol and guaiacol substrates with added CuSO4 , and on media with ABTS and ABTS with added CuSO4. T. virens LBKURCC70/ 71/85 did not show any laccase activity on day 3 of observation, but showed weak laccase activity on day 6 on media containing guaiacol and guaiacol with added CuSO4. The three T. virens LBKURCC70/71/85 fungi showed negative results when grown on media containing ABTS and ABTS with added CuSO4. From this study it can be concluded that the qualitative assay using PDA supplemented with laccase substrate indicators such as guaiacol or ABTS to screen for fungal producers of laccase is a good preliminary screen.
Keywords: Guaiacol, laccase , Trichoderma virens, Trichoderma asperellum, 2,2’-azino-di-3-etilbenzotiazol-6-sulfonat .

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 05. 220 (0015)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia., 2020

Deskripsi Fisik

xiii, 54 hlm,; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 05. 220 (0015)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

Lampiran Berkas

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