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Sekuensing Dna Fungi Kawasan Cagar Biosfer Giam Siak Kecil Bukit Batu Lbkurcc39 Menggunakan Sekuen Its Rdna Dan Analisis Filogenetik

Three isolates of LBKURCC37, LBKURCC38, and LBKURCC39, were fungi isolated and purified from peat soils originating from secondary forests in the Makmur Area of the Giam Siak Kecil Bukit Batu Nature Reserve (CAGSKBB), Riau. Soil samples are taken from the CAGSKBB core area. The results of previous studies found that LBKURCC39 produced chitinase in the highest amount above the chitinase activity produced by both LBKURCC37 and LBKURCC38 lines.
LBKURCC39 fungi have been morphologically identified and only identified at the genus level. Phylogenetic analysis is still needed to determine kinship at the species level. Before a phylogenetic analysis is carried out based on ribosomal DNA sequences in the ITS-1 and ITS-2 regions, optimization of DNA isolation and PCR ITS rDNA application are good for sequencing. Chromosomal DNA was isolated using the Wizard Genomic Purification ex Promega Co. (Madison, USA) kit from mycelia cells of three days old. LBKURCC39 fungal chromosomal DNA has a BM (molecular weight) of 11,102 bp. ITS rDNA was successfully amplified by PCR using ITS5 and ITS4 primer pairs, the annealing temperature was 420C and resulted in a PCR product with a molecular weight of 520 bp. The results of phylogenetic analysis of the ITS-1, ITS-2 and 5,8S rDNA regions of the LBKURCC39 fungi genome indicate that the species of LBKURCC39 fungi are Penicillium citrinum with 99% similar identity.
Keywords: Penicillium citrinum, Internal Transcribe Spacer, rDNA

Ketersediaan
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Perpustakaan Universitas Riau 10 05. 220 (0017)
10 05. 220 (0017)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
10 05. 220 (0017)
Penerbit
Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia.,
Deskripsi Fisik
xi, 41 hlm,; ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
10 05. 220 (0017)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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