CD Tesis
Desain Dan Pembuatan Sensor Dissolved Oxygen (Do) Berbasis Biochip-G Untuk Deteksi Polusi Air
Water pollution due to waste can reduce the level of water quality.
Preventive measures need to be taken to reduce the spread of the impact
of decreasing water quality. One of the water quality parameters is the
level of dissolved oxygen (Dissolved Oxygen / DO) contained in the water
system. The DO measurement method used is Biochip G-based Biosensor
that can perform DO measurements directly (real-time measurement) by
using biological components as Bio-transducers namely Algae Chlorella
Kessleri. The use of Alga Chlorella Kessleri is expected to provide a quick
response in detecting pollutants, namely the herbicide TASMIN 865SL
with variations in concentrations of 10% dan 50%.
The results produce a Biosensor module that is able to detect DO by
observing changes in the electrical potential detected by the analog circuit
signal processor and the biosensor module interface capable of displaying
measurements directly (real-time) and storing data for further data
processing. Initial module testing includes a Dummy test and the Biochip
test gives results according to the reference and shows the Biochip-G
electrode and biosensor module can work according to the Cellasys
GmbH standard. Testing the effect of lights was also carried out to
determine the effect of artificial lighting lamps directly on the electrodes
with empty Biochip conditions and contained the medium of Algae Culture
Broth (ACB) which showed the Biochip was only affected by contact with
the direct electrode in the form of liquid/solution.
The biosensor module is able to observe changes in DO as a result
of light/dark processes involving algal photosynthesis. Algae
photosynthesis events are characterized by changes in electrical potential
that correspond to changes in DO levels with a device sensitivity value of
0.17 mV/s. The effect of herbicide pollutant TASMIN 865SL can cause DO
production activity of Chlorella Kessleri algae to be decreased by changes
in the form of increasing potential values measured using Biosensor during
Algae photosynthesis, this potential increase occurs for each TASMIN
865SL herbicide concentration of 10% and 50% with a potential median
value measured for each concentration of 1907.37 mV and 1922.07 mV.
Key words: Algae, biosensors, biochip, herbicide waste, dissolved oxygen
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