CD Skripsi
Analisis Sekuens Barkode Dna Pada Daerah Trnv-Ndhc Intergenic Spacer Pada Tumbuhan Pandan (Benstonea Sp.) Asal Riau
Pandan (Benstonea sp.) from Riau is one of the plants that has an important role in the Kajuik Lake ecosystem located in Kampar River, Langgam, Pelalawan Regency, Riau Province. The DNA barcode analysis on pandan from Riau using sequence trnV-ndhC intergenic spacer was performed in this study. The steps of this research were sampling, total DNA extraction using Genomic DNA Mini Kit Plant (Geneaid), Polymerase Chain Reaction (PCR), electrophoresis, sequencing and data analysis. The data was analyzed using BioEdit versi 7.0, BLASTn, MEGA versi 6.0 programs. The result showed that the trnV-ndhC intergenic spacer obtained in this study was 1172 bp in size. Pandan from Riau was classified to genus Benstonea and no one species had similarity 100% to pandan from Riau based on GenBank database. Conclusion, pandan from Riau is suggested a new species never been identified and published.
Key words: Benstonea sp., DNA barcode, Genomic DNA Mini Kit Plant (Geneaid), Kajuik Lake, trnV-ndhC intergenic spacer.
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