CD Skripsi
Aktivitas Antibakteri Metabolit Sekunder Aktinomisetes Sebagai Penghasil Antibiotik Yang Diekstraksi Pelarut Etil Asetat
Infection from pathogenic bacteria can cause various diseases in humans, such as diarrhea and skin. The diarrheal disease can be caused by bacteria Escherichia coli, Salmonella typhimurium, and Shigella flexineri, while the cause of skin diseases caused by Staphylococcus aureus and Pseudomonas aeruginosa.Meanwhile, actinomycetes are reported to be one of the producers of antibiotics. The actinomycetes used were isolated from soil in the Universitas Riau Arboretum, namely isolates B1.06, B3.13, C2.18, A2.01, and C2.20. The purpose of this study was to study the antibacterial activity of secondary metabolites of Actinomycetes extracted using ethyl acetate against pathogenic bacteria that cause diarrheal and skin diseases. The disk diffusion method was used for testing antibacterial activity. From the results, it was found that the actinomycetes isolate was able to overcome the pathogenic bacteria, with a diameter of inhibition zone formed was 7.6 mm to 25.1 mm against Pseudomonas aeruginosa. The diameter of the inhibition zone formed was compared with the tetracylcline and produced antibacterial activity criteria of actinomycetes isolates that were resistant, intermediate and sensitive.
Key Words: Actinomycetes, antibiotic, ethyl acetate, extraction, secondary metabolites.
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