CD Skripsi
Isolasi Bakteri Penghasil Selulosa Dari Tanah Mangrove Dan Buah-Buahan Tropis Sebagai Biomaterial Untuk Produksi Kertas
Bacterial cellulose is a biopolymer produced by acetic acid bacteria through oxidative fermentation. Bacterial cellulose is used in various industrial fields, especially paper production due to its excellent physical-chemical properties. This study aimed to isolate potential bacteria producing cellulose from mangrove soil in Riau and tropical fruits (rambutan, matoa, apple) to be used as biomaterials for paper production. Bacterial isolation was carried out using 2 approaches, namely approach I (isolation, selection, production) and approach II (isolation, production). The isolation stage was carried out on Hestrin Schramm (HS) medium, the selection stage on Glucose Ethanol and Yeast Extract (GEY) medium and the production stage were carried out on HS production medium and modified production medium. There were 38 mixed cultures which are candidates for producing cellulose; however, none of the isolates was able to produce cellulose pellicle. The candidate isolates only produced a thin cellulose mat floating on the medium. The isolates producing cellulose mats were obtained from matoa 3, rambutan 1, 2, 3, and mangrove B2.
Keywords: Bacteria producing cellulosa, cellulose pellicle, cellulose mat.
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