Image of Analisis Produksi Lakase Secara Fermentasi Padat Batang Padi Oleh Trichoderma Asperelloides Lbkurcc2, Trichoderma Virens Lbkurcc71 Dan Trichoderma Virens Lbkurcc85
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Analisis Produksi Lakase Secara Fermentasi Padat Batang Padi Oleh Trichoderma Asperelloides Lbkurcc2, Trichoderma Virens Lbkurcc71 Dan Trichoderma Virens Lbkurcc85

Laccase is an oxidoreductase enzyme that are known to catalyze the oxidation of aromatic substances along with the reduction of oxygen molecules to water. Previous studies have shown that the fungus Trichoderma sp. can produce the laccase enzyme. The purpose of this study was to determine the growth curve and quantitatively analyze the ability of laccase production by solid state fermentation (SSF) of rice straw by Trichoderma asperelloides LBKURCC2, Trichoderma virens LBKURCC71 and Trichoderma virens LBKURCC85. SSF of rice straw was done for 8 days, by incubating a mid-log phase inoculum of the fungi tested with an SSF media containing rice straw. The inoculum was prepared by inoculation of 30 mL PDB media with 2 plugs of the test fungi grown on PDA, and incubated by shaking at room temperature (30oC). Incubation time needed to reach mid-log phase of growth in this inoculum for T. asperelloides LBKURCC2 and T. virens LBKURCC85 was 2 days, and for T. virens LBKURCC71 was 3 days. Crude extracellular enzymes that were produced by SSF of rice straw were extracted from the media and washed using 10 kDa ultrafiltration membranes. The laccase enzyme activity assay was performed with a substrate of 2,2-azinobis (3-ethylbenzatiazoline) -6-sulfonate (ABTS) and incubated for 0, 5 and 10 minutes. After incubation, absorbance was measured using a UV-Vis spectrophotometer with a wavelength of 420 nm. The absorbance obtained at times t was compared to the absorbanceat times 0. The results showed that T. asperelloides LBKURCC2 could produce laccase by SSF of rice straw, with an activity of (370.8 ± 88.8) U/L, while no laccase activity was produced by T. virens LBKURCC71 and LBKURCC85. 

Ketersediaan
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Perpustakaan Universitas Riau 03 02. 120 (0049)
03 02. 120 (0049)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 02. 120 (0049)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Kimia.,
Deskripsi Fisik
xiii, 47 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 02. 120 (0049)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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