CD Skripsi
Isolasi Dan Uji Antioksidan Senyawa Metabolit Sekunder Dari Ekstrak Diklorometana Tumbuhan Paku Ahaka (Vittaria Elongata Sw)
Paku Ahaka (V. elongata) is an epiphytic plant which is included in the family of Pteridaceae which typically grows on palm trees and wetlands. It has been reported that some species from this genus have cytotoxic and antioxidant activities, but its secondary metabolites and biological activities have not been reported in V. elongata. The work therefore aimed to distinguish the secondary metabolites and antioxidant activity from the extract of V. elongata dichloromethane. The methanol extract was obtained by using maceration (3 x 24 h). The isolation of secondary metabolites was carried successively with partition with different organic solvents (n-hexane, dichloromethane, and ethyl acetate), Thin Layer Chromatography (TLC) and Vacuum Liquid Chromatography (VLC). Crystalline was formed from fraction 7 (n-hexane: ethyl acetate; 4:6) and recrystalized to obtain white crystal, provided with a code VE-DCM-01. The purity of the compound was conducted with melting point and resulting 182–184oC, High Performance Liquid Chromatography (HPLC) showed one intense peak at 254 nm. The compound was characterized by using FTIR and showing the presence of the aromatic group C - O (ester), C= C, C = O, aliphatic group C-H, C-H, and OH. In addition, the antioxidant activity analysis on the compound, n- hexane, dichloromethane, ethyl acetate, and water extracts was performed using 1,1-diphenyl-2-picrylhidrazil (DPPH) method. Dichloromethane, ethyl acetate, and water extract showed antioxidant activity with IC50 value of 472.35 μg / mL, 94.72 μg / mL, and 13.63 μg / mL, while n-hexane extract and compound VE-DCM-01 showed no antioxidant activity, IC50 > 1000 μg / mL.
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