CD Skripsi

Analisis Sekuens Dna Penyandi 18s Rrna Pada Tumbuhan Cocor Bebek (Kalanchoe X Laetivirens)

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ABSTRACT
Plant stress studies are based on gene expressions. This research aims to analyze the DNA sequence of 18S rRNA in cocor bebek (K. x laetivirens). The research methods being out are total DNA isolation, electrophoresis, PCR, sequencing and data analysis utilizing BioEdit, BLASTn and Mega 6 programs. Partial DNA sequence of 18S rRNA in K. x laetivirens has been obtaine with 419 bp length. The DNA sequence has 99,28% similarity to K. daigremontiana. Kalanchoe x laetivirens forms one group with another species from the same family based on the DNA sequence of 18S rRNA. A primer pair specific to K. x laetivirens for amplifying 18S rRNA has been designes such as forward 5’- CAA ATT ACC CAA TCC TGA CA -3’ and reverse 5’- CCA ACG TAA ATA GGA TCG AA -3’. The partial DNA sequence of 18S rRNA in K. x laetivirens is potentially being employed as an internal control once the validation process completed.
Keywords: 18S rRNA, housekeeping gene, internal control, Kalanchoe x laetivirens

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 04. 121 (0014)

Penerbit

Pekanbaru : Universitas Riau - Fakultas Matematika Dan Ilmu Pengetahuan Alam - Biologi., 2021

Deskripsi Fisik

xiv, 74 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 04. 121 (0014)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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