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Isolasi Bakteri Penghasil Polihidroksi Alkanoat Dari Tanah Gambut Dan Tanah Tempat Pembuangan Akhir Sampah Di Riau

ABSTRACT
Conventional plastics are difficult to degrade and can pollute the environment. Therefore, another alternative to replace conventional plastic is needed by using bioplastics which are more biodegradable. The purpose of this study was to obtain potential bacterial isolates to produce Polyhydroxy Alkanoate (PHA) as a bioplastic feedstock from peat soil and landfills in Riau. PHA producing bacteria were isolated using Nutrient Agar medium with the spread method. PHA producing bacteria were selected using Sudan Black dye and indicated with blackish blue. The selected bacterial isolates were cultured on mineral salt medium for the production of PHA and the resulting PHA was extracted using chloroform. A total of 43 from 182 bacterial isolates obtained from four sampling locations were able to produce PHA. The reselection results showed that 16 of the isolates showed consistency in their ability to produce PHA which was indicated by the absorption of sudan black staining by the bacterial colony. Cell dry weight of the selected isolates ranged from 0,3-2,20 g/L, dry weight of PHA ranged from 0,03-0,43 g/L, biomass residues ranged from 0,16 to 2,17 g/L, and PHA accumulation ranged from 1,17% -63,58%. The highest PHA accumulation was produced by TPAS2.1 isolates obtained from landfills and the lowest by KB2.3 isolates from orchards.
Keywords: Conventional Plastic, Polyhydroxy Alkanoate

Ketersediaan
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Perpustakaan Universitas Riau 03 04. 121 (0015)
03 04. 121 (0015)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 04. 121 (0015)
Penerbit
Pekanbaru. : Universitas Riau - Fakultas Matematika Dan Ilmu Pengetahuan Alam - Biologi.,
Deskripsi Fisik
ix,112 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 04. 121 (0015)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
BIOLOGI
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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