CD Skripsi
Respon Daun Tacca Chantrieri Terhadap Pembentukan Kalus Dengan Berbagai Konsentrasi 2,4-D Dan Bap Secara In Vitro
ABSTRACT Tacca chantrieri is a species of the Tacca genus from the Dioscoreaceae family which is an annual herbaceous tropical plant. T. chantrieri has a unique inflorescence morphology like a bat. T.chantrieri rhizome contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, saponins, and has played a role as traditional medicine in Southeast China and Thailand. The rampant action of land clearing and forest conversion resulted in the number of T.chantrieri decreasing. This encourages multiplication to maintain its sustainability. T. chantrieri can be propagated through in vitro culture techniques as callus culture. The purpose of this study to determine the response of T.chantrieri leaf callus formation and determine the optimal concentration with various concentrations of 2,4-D and BAP by in vitro. This study used a completely randomized design with 7 levels of treatment and observation for 60 days after planting. The results showed that the concentration of 1.5 mg / L 2,4-D + 3 mg / L BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared at 18,75 days after planting. Keywords: Tacca chantrieri, secondary metabolites, in vitro, callus, 2,4-D, BAP.
Tidak tersedia versi lain