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Seleksi Dan Uji Aktivitas Selulase 24 Isolat Bakteri Endofit Dari Akar Mangrove Ceriops Tagal (Perr) C.B. Rob

ABSTRACT
Endophytic bacteria from mangroves have the potential to produce cellulase enzymes. The application of cellulase enzymes in vivo and in vitro is relatively large, so that research to obtain isolates capable of producing cellulase enzymes with high activity is still ongoing. Collection of 48 isolates from local strains of mangrove roots Ceriops tagal (Perr) C. B. Rob. (C. tagal) its potential to produce cellulase is unknown By Biochemistry of Laboratory, Department of Chemistry Faculty of Mathematics and Natural Sciences, University of Riau (FMIPA UNRI). In this study, only 24 isolates were selected from 48 isolates qualitatively using Carboxymethyl Cellulose (CMC) 1% selective solid media, as well as determining the cellulase activity produced quantitatively using the Dinitro Salicylic Acid (DNS) method. The results of the selection of 24 endophytic bacterial isolates from mangrove roots of C. tagal on CMC 1% selective solid media obtained 22 live isolates because they were able to produce extracellular cellulase enzymes and 2 non-living isolates. Cellulase activity data from 22 isolates were statistically tested using Duncan’s Multiple Range Test (DNMRT) method at a significance level of 5% and Principal Component Analysis (PCA), it showed that LBKURCC332 isolate (572±69) x 10-4 U/mL had the highest cellulase activity in the 1st cluster and was significantly different from other isolates. The lowest cellulase activity LBKURCC336 isolate (12±4) x 10-4 U/mL in the 7st cluster and not significantly different from LBKURCC isolates (344 and 339).
Keywords: Cellulase, CMC, endophytic bacteria.

Ketersediaan
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Perpustakaan Universitas Riau 03 02. 121 (0003)
03 02. 121 (0003)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 02. 121 (0003)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Kimia.,
Deskripsi Fisik
ix,112 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 02. 121 (0003)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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