CD Skripsi

Analisis Tiga Metode Penentuan Aktivitas Enzim Α-Amilase

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SUMMARY
α-amylase enzyme is a biocatalyst in the process of hydrolyzing starch into reducing sugar molecules (maltose). The enzyme activity as a biocatalyst can be calculated based on the number of moles of the substrate that is hydrolyzed into product per unit time, or the number of moles of product produced per unit time. The method used to measure the number of moles of starch substrate hydrolyzed is the Iodine method, while for the number of moles of reducing sugar products from amylase hydrolysis by amylase using the 2,3-dinitrosalicylic acid (DNS) method and the Nelson-Somogyi (NS) method. This third method are with experimental conditions of pH 7, temperature of 50°C and an incubation time of 30 minutes. The results showed that the α-amylase enzyme activity measured by the DNS method was 0.115 ± 0.002 μmol/minute and the NS method was 0.041 ± 0.025 μmol/minute, while the Iodine method had activity of 2.090 ± 0.005 μmol/minute. The activity data of these three methods after being statistically tested by the Duncan method turned out to be significant between the DNS method (2,3-dinitrosalicylic acid) with the Nelson-Somogyi (NS) method and the Iodine method.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 121 (0011)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2021

Deskripsi Fisik

ix,112 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 121 (0011)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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