CD Skripsi

Penentuan Aktivitas Enzim Lakase Trichoderma Asperellum Lbkurcc1 Yang Telah Difraksinasi Secara Parsial Menggunakan Amonium Sulfat

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SUMMARY
Laccase are extracellular enzymes containing metal ions which used oxygen compounds to catalyze the oxidations process of various aromatic and nonaromatic compounds. The purpose of the study was to separating laccase enzyme and determining laccase spesific activity from the isolate fungus T. asperellum LBKURCC1. Laccase enzymes fractination process was carried out by two steps, that was fractination saturated with the addition of ammonium sulfate salts with saturation variations of 0-20%, 20-40%, 40-60% and 60-80%, and fractionation with ultrafiltraton membranes 10.000 MWCO (Molecular Weight Cut Of). Based on this research, the results obtained were the activity of crude extract laccase of 0,019 U/L, and highest enzymes activity was the enzyme purified by ammonium sulfat salt 0-20% saturation fraction of 0,030 U/L. Specific activity of crude extract laccase enzyme was obtained at 4,435 U/mg and the specific activity of purified enzymes decreased 2,196 U/mg with a yield of 157,129%. The results showed that enzymes in the saturation fraction of 0-20% ammonium sulfate was high activity and purity.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 121 (0015)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2021

Deskripsi Fisik

v, 107 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 121 (0015)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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