CD Skripsi
Isolasi Metabolit Sekunder Dan Uji Toksisitas Ekstrak Etanol Dari Daun Bakau Rhizophora Mucronata (Rhizophoraceae)
SUMMARY
Rhizophora mucronata is a type of mangrove that has a variety of secondary metabolite
compounds. Secondary metabolites in Rhizophora mucronata include alkaloids,
flavonoids, tannins, and other phenol compounds. The purpose of this study is
secondary metabolite isolation of mangrove leaves Rhizophora mucronata and toxicity
test using BSLT method. This research was carried out using a maceration method using
ethanol. Ethanol crude extract as much as 97.36 g is done separation with
chromatography of gravity column obtained in the form of dirty crystals on vials 50-70
and crystallized to get a compound codenamed Rm-FE in the form of white crystals
weighing 13.57 mg with a melting point of 172-174 ºC. Phytochemical test results of
Rm-FE compounds are positive for flavonoid compound groups. Rm-FE UV spectrum
indicates the presence of absorption at λmax 274 nm. The FT-IR spectrum shows peaks
at waves of 1053-1141 cm-1 (C-O), 1606-1635 cm-1 (C=C), 3309 cm-1 (O-H), and
2912–2936 cm-1 (C-H aliphatic). Toxicity test results with BSLT (Brine Shrimps
Lethality Test) method against ethanol coarse extract from Rhizophora mucronata
leaves are non-toxic.
Keywords: R. mucronata, secondary metabolities, toxicity
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