CD Skripsi
Isolasi Senyawa Metabolit Sekunder Dan Uji Toksisitas Ekstrak Etil Asetat Dari Daun Rhizophora Mucronata (Rhizophoraceae)
ABSTRACT
Rhizophora mucronata is a type of mangrove that has diverse secondary metabolites. Secondary metabolites in Rhizophora mucronata include alkaloids, flavonoids, tannins and phenol compounds. The purpose of this study was to isolate secondary metabolites and to determine toxicity test by BSLT method. Leave extraction was carried out using warm water and then extracted with ethyl acetate. The extraction result after being evaporated with rotary evaporator obtained a crude extract of 3,95 g dark green. Crude ethyl acetate extract was separated using liquid vacuum chromatography and 6 fractions produced. Point crystals were isolated from first fraction of ethyl acetate with melting poin of 218-220 oC. The isolated compound was coded as RmEF1. The crystals were characterized using UV and FT-IR spectroscopy. UV spectrum of RmEF1 indicated absorption at λmax 311 nm. The FT-IR absorption spectrum showed absorption peaks at 1037 (C-O), 1384 (C-H) methyl,1472 (C-H) methylen, 1606 (C=C), and 2934 (C-H aliphatic). From the Lieberman Burchard test, the RmEF1 compound is a terpenoid class. The result of toxicity test by BSLT (Brine Shrimps Lethality Test) method on that crude extract was non-toxic.
Keyword: rhizophora mucronata, secondary metabolites, toxicity
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