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Produksi Dan Fraksinasi Kromatografi Kolom Metabolit Sekunder Berpotensi Antibakteri Escherichia Coli Dari Penicillium Sp. Lbkurcc34

SUMMARY
Penicillium sp. LBKURCC34 is a collection isolate of Biochemistry Laboratory, Department of Chemistry. This isolate is originally from Biosphere Reserve in Giam Siak Kecil Bukit Batu (GSKBB) Province of Riau. The fungus produces potential secondary metabolites that are able to inhibit Escherichia coli. The purpose of this study was to purifiy ethyl acetate extract of the liquid media of Penicillium sp. LBKURCC34 and to analyse the antibacterial against E. coli of it is fraction. Production of secondary metabolites was carried out by fermentation in liquid media which was inoculated with Penicillium sp. spores LBKURCC34 (7 x 1012 spores for 50 mL media). Fermentation was carried out for 14 days and extracted with ethyl acetate. The extract was evaporated, then separated by column chromatography by using SiO2. Antibacterial tests were performed by the disc diffusion method toward E. coli with a concentration of 57 μg/μL. Amoxicillin was used as positive controls and methanol as solvent controls. Further identification of crude extract was tested by TLC by comparing the eluent ethyl acetate: n-hexane (1:1; 3:2; and 7:3) with UV light (254 and 366 nm) HPLC analysis of crude extract showed 7 peaks (360 nm) and 5 peaks (254 nm). Column chromatography separation produced 13 fractions. In the antibacterial analysis, it was found that fractions 10 (F10), 11 (F11) and 13 (F13) exhibited antibacterial activity with a clear zone highlight 6,14 mm. These 3 fractions were the most effective antibacterial

Ketersediaan
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Perpustakaan Universitas Riau 03 02. 121 (0022)
03 02. 121 (0022)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 02. 121 (0022)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Kimia.,
Deskripsi Fisik
ix,112 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 02. 121 (0022)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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