CD Skripsi

Kemampuan Produksi Lakase Trichoderma Sp. Lbkurcc22 Dan Lbkurcc70 Secara Fermentasi Padat Batang Padi Dan Kayu Keruing

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SUMMARY
Laccase is an oxidoreductase enzyme that is able to oxidize phenolic compounds
and use oxygen as an electron acceptor. Laccase enzymes can be produced by
fungi such as Trichoderma sp. Laccase produced by fungi can be produced on
solid media or Solid State Fermentation (SSF). Rice straw and keruing wood
sawdust (Diptorocarpus sp.) are waste containing lignocellulose which can be
used as a fermentation medium for laccase production. This research aims to
determine the growth curve and analyze the laccase enzyme activity of
Trichoderma aureoviride LBKURCC22 and Trichoderma virens LBKURCC70
produced on solid fermentation media of rice straw and keruing wood sawdust.
The fermentation process was carried out for 8 days by incubating the fungal
inoculum which was in its mid-exponential phase. The fermentation medium was
then extracted using acetate buffer 0.05 M pH 5.5. The crude extract of the
enzyme obtained was then washed with an acetate buffer of 0.05 M pH 5.5 using a
10 kDa ultrafiltration membrane. Laccase enzyme activity test was carried out
using a UV-Vis spectrophotometer at a wavelength of 420 nm and incubated for
0, 5 and 10 minutes using 2,2-azinobis (3-ethylbenzatiazoline) -6-sulfonate
(ABTS) as a substrate. Laccase enzyme activity was statistically tested through
the t test at a confidance level of 5%. Laccase activity of both fungi was only
obtained in production using rice straw. Laccase activity produced on rice straw
was 13,875 ± 7,825 U / L for T. aureoviride and 9,025 ± 9,158 U / L for T. virens
LBKURCC70. Based on Student's t-test, it was obtained t count < t table, so there
was no significant difference between the laccase activity of T. aureoviride
LBKURCC22 and T. virens LBKURCC70 which was produced by solid state
fermentation of rice straw.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 121 (0023)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2021

Deskripsi Fisik

v, 107 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 121 (0023)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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