CD Skripsi
Seleksi Dan Uji Aktivitas Selulase 16 Isolat Bakteri Endofit Dari Akar Mangrove Bruguiera Sp
SUMMARY
Cellulase enzymes are enzymes that have many benefits both in vitro and in vivo. In vitro, this enzyme is used as a biocatalyst for certain reactions in industry, while in vivo it can be used to convert cellulose waste into products of economic value. In thisstudy, 16 isolates were selected from 47 endophytic bacterial isolates from the mangrove roots of Bruguiera sp, which are a collection of the Biochemistry Laboratory of FMIPA UNRI. The selection was carried out in selective solid media containing 1% carboxymethyl cellulose (CMC), while the activity of the crude extract of the enzymes produced in liquid media was determined using the 3,5- dinitrosalicylic acid (DNS) method. The results of the selection of 16 endophytic bacteria from mangrove roots Bruguiera sp in 1% selective medium CMC obtained 15 live isolates because they were able to produce extracellular cellulase enzymes and 1 non-living isolate. Cellulase activity based on statistical tests with the DMNRTmethod at 5% level and PCA obtained 2 isolates in the first cluster with relatively high cellulase activity from other isolates and not significantly different, namely LBKURCC356 with an activity of (293.10-4 ± 11.10-4) U / mL and LBKURCC360 at (266.10-4 ± 25.10-4) U / mL. The lowest cellulase activity in the 6th cluster was LBKURCC330 with cellulase activity of (25.10-4 ± 8.10-4) U / mL.
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