CD Skripsi

Isolasi Dan Uji Antibakteri Senyawa Metabolit Sekunder Ekstrak Etil Asetat Dari Tumbuhan Kelakai (Stenochlaena Palustris)

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SUMMARY
Stenochlaena palustris is a species of the genus Stenochlaena that grows in swampy are as and around palm trees, where it frequently forms dense thickets and spreads to cover the ground. Numerous species in this genus have been reported to be antioxidants and antibacterial agents. The purpose of this study is to isolate secondary metabolites from ethyl acetate extract and to evaluate antibacterial activity of the extracts. The maceration method was used in this study, followed by partitioning with ethyl acetate solvent to obtain ethyl acetate extract. Flash chromatography was used to separate up to 3 g of ethyl acetate extract, resulting in crystals in vials 50 and 51. The crystals were then recrystallized, yielding a pure compound coded SP-EA-01 in the form of white crystals weighing 5 mg and witha melting point of 140-142°C. In the Liebermann-Burchard (LB) test, the crystals were positive for the terpenoid group. SP-EA-01 exhibited no absorption in the UV-Vis spectrum but did exhibit peaks in the FTIR spectrum between wave numbers 2873-2960 cm-1 and 1027-1160 cm-1 aliphatic (C-H) and ether (C-O-C). Antibacterial activity was determined using the agar diffusion technique. The extracts of S. palustris were used at a concentration of 50 μg, the antibiotic amoxsan® was used as a positive control, and dimethyl sulfoxide (DMSO) was used as a negative control. The results indicated that the ethyl acetate extract was capable of inhibiting Escherichia coli and Staphylococcus aureus growth by 6.1 mm and 7.1 mm, respectivel.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 121 (0030)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2021

Deskripsi Fisik

ix,112 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 121 (0030)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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