CD Skripsi
Diferensiasi Leukosit Ikan Lele Dumbo (Clarias Gariepinus) Yang Diberi Pakan Mengandung Kunyit (Curcuma Domestica Val.) Dan Diinfeksi Aeromonas Hydrophila
ABSTRACT
This research was conducted from August to October 2020 at the Laboratory of Fish
Parasites and Diseases, Department of Aquaculture, Faculty of Fisheries and Marine,
University of Riau. This study aims to obtain the best dose of feeding containing turmeric
(Curcuma domestica Val.) in african catfish (Clarias gariepinus) to prevent Aeromonas
hydrophila infection. The method used is an experimental method using a Completely
Randomized Design (CRD) one factor five treatment levels and 3 replications. The
treatments applied were Kn (control, no turmeric), Kp (no turmeric and tested against with A.
hydrophila), P1 (0.5 g turmeric per kg of pellets and tested against with A. hydrophila), P2
(0.7 g/kg), P3 (0.9 g/kg). Fish were reared for 45 days in a 40x30x30 cm aquarium with a
stocking density of 1 fish/3L of water. The challenge test was carried out on the 31st day
using A. hydrophila with a density of 108 CFU/ml. After the challenge test, the fish were
reared again until the 45th day. Kp and P1 treatments showed clinical symptoms with excess
mucus, skin lession, abnormal movement, exopthalmia, low appetite, and fin flakes. Feeding
containing turmeric (C. domestica Val.) has a significant effect to prevent A. hydrophila. The
best dose is 0.9 g/kg which was marked by a total leukocytes of 11.29x104 cells/mm3, a
leukocytes levels of 1.33%, a lymphocytes of 79.66%, a monocytes of 12.00%, a neutrophils
of 7.00% , a phagocytosis activity of 34.66%, an absolute weight growth of 7.2 g, and a fish
survival of 80.00%.
Key words : African Catfish, Aeromonas hydrophila, Turmeric, Hematology, Leukocytes
1) Students of the Faculty Fisheries and Marine, University of Riau
2) Lecturers of the Faculty Fisheries and Marine, University of Riau
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