CD Skripsi
Bakteri Penghasil Bioplastik Dari Limbah Industri Sagu Dan Tahu Di Riau: Isolasi, Seleksi, Dan Optimasi Produksi
Conventional plastic is difficult to degrade and can pollute the environment.
Therefore, an alternative to conventional plastic is needed by using bioplastics
that are more easily biodegradable. The purpose of this study was to obtain
potential bacterial isolates to produce PHA as raw material for bioplastics from
industrial waste of sago and tofu in Riau. PHA-producing bacteria were isolated
using Nutrient Agar (NA) medium with the spread plate method. Qualitatively,
PHA-producing bacteria were selected based on their ability to absorb the Sudan
black solution. The selected bacterial isolates were cultured in mineral salt
medium (MSM) for the production of PHA and the resulting PHA was extracted
using chloroform. A total of 5 isolates that showed high color absorption intensity
were isolated from sago factory waste located in Goguk Village, Meranti Islands.
The highest PHA accumulation was produced in 50% LCPKS medium by LPS1_9
isolate, while the lowest PHA accumulation was produced in MSM medium by
LPS1_12 isolate. The use of 50% LCPKS as a carbon source tends to support an
increase in the accumulation of PHA, compared to the use of a carbon source
from a minimal nutrient medium MSM.
Keywords: Conventional Plastic, Sudan Black, Polyhydroxy Alkanoate,
Chloroform
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