CD Skripsi
Isolasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Dari Ekstrak N-Heksana Tumbuhan Paku Pyrrosia Longifolia
Pyrrosia longifolia is a fern species in the Polypodiaceae family. This species is
frequently found attached to old trees, usually on branches or nagging branches.
The goal of this study was to isolate secondary metabolites of P. longifolia and
analyze their toxicity using the Brine Shrimp Letalithy Test (BSLT). This species
was extracted using the maceration method with methanol and fractionated with
n-hexane, dichloromethane, and ethyl acetate. In addition, the n-hexane extract
was purified using liquid vacuum chromatography, yielding 11 fractions. A
crystalline solid was discovered in fraction 4, and recrystallization was performed
to obtain a white crystal, which was labeled as 20-05-A-F4. The purification of
20-05-A-F4 was analyzed using thin layer chromatography (TLC) and a melting
point of 124-126oC. UV/Vis and FTIR spectrophotometers were used to
characterize the compound. Peaks in FTIR spectroscopy were found at 2960cm-1
(aliphatic CH), 2850 cm-1 (aliphatic CH), 1465 cm-1 (CH in CH2), and 1382 cm-1
(CH in CH3). The toxicity analysis of n-hexane revealed that it was not toxic, as
were the fractions.
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