CD Skripsi
Identifikasi Senyawa Metabolit Sekunder Dari Fraksi Ekstrak Rumput Laut Cokelat (Sargassum Plagyophyllum)
ABSTRACT
Seaweed has potential as a source of bioactive compounds, food and
medicine. One of the seaweeds that has the potential to be used for its bioactive
compounds is the brown seaweed Sargassum plagyophyllum. Fractionation is a
technique of separating and grouping the chemical content of extracts based on
their polarity. This study aims to determine the bioactive components (secondary
metabolites) of flavonoids, alkaloids, steroids/terpenoids, saponins and phenols
qualitatively and quantitatively from the extract of S. plagyophyllum by
fractionation method. The research method used was experimental. S.
palgyiophyllum was extracted using the fractionation method. The treatment given
by different polarity solvents consisted of methanol, was done fractionation with
using n-hexane, ethyl acetate, and butanol. Extraction of samples the first used
methanol, after that methanol extract was diffracted with n-hexane, ethyl acetate
and butanol. Parameter analysis was consisted of phytochemical test (qualitative
analysis) and quantitative analysis of total phenol, flavonoid, and saponin content.
The results showed that qualitative testing of the secondary metabolites of S.
plagyophyllum contained in the methanol extract were steroids/terpenoids,
saponins and phenols, the positive hexane fraction contained steroids/terpenoids,
saponins, the ethyl acetate fraction positively contained alkaloids, flavonoids,
steroids/terpenoids, and saponins. and phenol while the butanol fraction contains
steroids/terpenoids, saponins. While quantitative secondary metabolites were
tested on the hexane fraction (0.18% saponins), then the ethyl acetate fraction
(0.14% flavonoids, 0.11% saponins, 0.12% total phenol) and butanol fraction
(0.05% saponins).
Keywords: qualitative , quantitative, polarity of solvents, saponin, flavonoid, total
phenol.
1) Student at Faculty of Fisheries and Marine Science, Universitas Riau
2)Lecturer at Faculty of Fisheries and Marine Science, Universitas Riau
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