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Pengaruh Mutagen Etil Metan Sulfonat (Ems) Terhadap Pertumbuhan Dari Eksplan Mata Tunas Philodendron Patriciae Secara In Vitro
Philodendron patriciae is one of the beautiful leafy ornamental plants. This plant
can absorb air pollution in the room. Sales of P. patriciae plants often experience
a decrease in price and propagation of seedlings that take a long time. The in vitro
mutation induction method is a plant seed propagation technique and a method to
increase the variety of these plants by using a chemical mutagen, Ethyl Methane
Sulphonate (EMS). The purpose of the study was to find out and determine the
proper concentration of EMS to produce the expected mutant P. patriciae plant
with permanent properties. This research was conducted at the Integrated Biology
Laboratory, Department of Biology FMIPA UNRI from June to September 2021
using a Complete Random Design (RAL) consisting of 7 treatment levels, 5
repeats with 35 experimental units. As for the treatment used as follows, namely
(K0) control; (K1) 0.025; (K2) 0.050; (K3) 0.075; (K4) 0,1; (K5) 0.125; (K6)
0.150 ml/20 ml EMS. The results obtained in this study are the time of appearance
and the highest shoot height is found in the K6 treatment with an average of 12.20
HST and 1.52 cm. The percentage of explans forms buds and the highest number
of leaves is found in the K3 treatment with an average of 5.60 buds and 3.40 leaf
strands. Changes in the morphology of the explan were seen in the treatment of
K3, K5 and K6 with smaller shoot sizes, the base of the shoots swelled, the leaves
were smaller, the stalks of long and slender leaves, and white haired roots with
varying root sizes.
Keywords: Ethyl Methane Sulphonate (EMS), In Vitro, Mutation, Philodendron
patriciae
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