CD Skripsi

Analisis Sekuens Barkode Dna Pada Gen Matk Tumbuhan Terung Asam (Solanum Ferox L.)

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DNA barcode is a technique using short and standardized sequences of
DNA which aims to speed up and simplify the process of identifying a species.
This study used DNA barcodes from the matK gene to analyze the DNA barcode
sequences of sour eggplant originating from Kampar. Sour eggplant is one of the
plants of the genus Solanum, totaling about 1.400 species distributed in tropical
and subtropical areas with a wide distribution of habitats. The research stages
included morphological observations, sample preparation, isolation using the
Geneaid kit, total DNA electrophoresis, PCR using the DreamTaq DNA
polymerase kit (Thermo Scientific), electrophoresis of PCR products, sequencing,
and data analysis using the BioEdit, BLASTn and MEGA version 6.0 programs.
The results showed that the sour eggplant studied had a DNA sequence length of
the matK gene of 818 bp. Morphologically, sour eggplant has a special
characteristic, namely it has fine hairs on the entire surface of the body.
Molecularly, the studied eggplant has similarities with S. rostratum, but the
morphological characteristics of the two are different from each other. The highest
identity value, which is 98.54%, was found in S. rostratum. Dendrogram,
indicating that the sour eggplant studied formed a group with S. rostratum species.
There were 25 nucleotide variations among the accessions studied and 10 of them
were critical nucleotides. These differences are caused by substitution mutation.
In this study, accessions with an identity value of 100% have not been found. This
sequence is the DNA sequence of the matK gene first reported in sour eggplant
(Solanum ferox L.).
Key Word: DNA Barcode, Sour Eggplant, Solanum ferox L., matK, PCR.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 04.121 (0078)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Biologi., 2021

Deskripsi Fisik

xii, 46 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 04.121 (0078)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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