CD Skripsi

Analisis Sekuens Barkode Dna Pada Daerah Trnl-Trnl-Trnf Intergenic Spacer Tumbuhan Kabau (Archidendron Microcarpum)

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DNA barcoding is a molecular identification technique of organisms that
uses short DNA sequences or DNA barcodes. The DNA barcode commonly used
in molecular analysis is the trnL-trnL-trnF intergenic spacer which has a higher
variation and mutation rate than rbcL and matK. This study aimed to analyze
DNA sequences in the trnL-trnL-trnF intergenic spacer area of kabau
(Archidendron microcarpum) plants taken from Ukui, Ukui District, Pelalawan
Regency. This study consisted of several stages, namely morphological
observations, sample preparation, total DNA isolation, total DNA electrophoresis,
PCR, electrophoresis of PCR products, and data analysis using software such as
Bioedit, BLASTn, and MEGA. The results showed that the DNA sequence of the
trnL-trnL-trnF intergenic spacer in kabau plants obtained a size of 879 bp and
had the highest similarity with Pithecellobium clypearia (97.61%). There were 12
nucleotide variations among the accessions studied and two of them were critical
nucleotides. Kabau plants form a group with Pithecellobium clypearia and
Archidendron lucyi. Based on this study, no 100% identity value was found,
which means that the DNA sequence trnL-trnL-trnF intergenic spacer from kabau
plants does not yet exist in the Genbank database.
Keywords: Archidendron microcarpum, DNA barcode, kabau plant, trnL-trnLtrnF
intergenic spacer, Ukui.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 04.122 (0003)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Biologi., 2022

Deskripsi Fisik

xii, 41 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 04.122 (0003)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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