CD Skripsi

Analisis Sekuens Barkode Dna Pada Daerah Trnl-Trnl-Trnf Intergenic Spacer Tumbuhan Jengkol Buah Besar (Archidendron Pauciflorum)

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DNA barcode is one of the technique used for molecular identification
with standardized short DNA sequences. In this study, the sequences used for
DNA barcode analysis in jengkol (Archidendron pauciflorum) were from the
trnL-trnL-trnF Intergenic Spacer region. The trnL-trnL-trnF Intergenic Spacer
region was a noncoding region derived from the chloroplast genome.
Archidendron pauciflorum belongs to the Fabaceae family which is identical to
the shape of the pod. This study used Archidendron pauciflorum from 3 different
individuals taken from Danau Bingkuang, Kampar District, Kampar Regency. The
stages of this research include morphological observations, total DNA isolation,
total DNA electrophoresis, PCR, electrophoresis of products PCR and data
analysis. Data analysis used several programs, such as Bioedit, BLASTn and
MEGA. The results showed that the sequence size of the trnL-trnL-trnF
Intergenic Spacer region in the Archidendron pauciflorum was 874 bp. In this
study, the percentage of identity value was not found to be 100%. The highest
percentage of identity value was found in the Archidendron lucyi with a value of
98.86%. This indicates that the Archidendron pauciflorum sequence is not yet
available in the genbank database.
Keywords: Archidendron pauciflorum, Barcode of DNA, Cloroplas genom,
Fabaceae, noncoding, trnL-trnL-trnF Intergenic Spacer, Kampar
Regency.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 04.122 (0004)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Biologi., 2022

Deskripsi Fisik

xii, 42 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 04.122 (0004)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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