CD Skripsi
Seleksi Bakteri Asam Laktat Lokal Penghasil Eksopolisakarida Dan Optimasi Produksi Pada Variasi Suhu, Waktu Inkubasi Serta Medium Fermentasi
Exopolysaccharides (EPS) are secondary metabolites produced by
microorganisms, EPS is produced by lactic acid bacteria (LAB) under unfavorable
conditions. EPS is widely used in the health and food sector. The purposes of this
study were to select LAB capable of producing EPS and to determine the optimal
time, temperature, and medium required by LAB to produce EPS.
This study used 5 isolates, JN2, JN5, JN6, S3PP8, and S3PP12. The
research consisted of two stages, selection of LAB for EPS production and EPS
production test with variations in temperature, incubation time, and fermentation
medium. In the selection stage of LAB producing EPS in 2% skim milk liquid
media, the five isolates did not produce exopolysaccharides. Selection results
using MRSA added with sucrose showed that 1 isolate (JN6) produced mucoid
exopolysaccharide, while 4 isolates (JN2, JN5, S3PP8 and S3PP12) produced
mucoid and ropy type exopolysaccharides. EPS production test of S3PP12 isolates
on MRSB-glucose and MRSB-sucrose medium showed that LAB could not
produce EPS.
Keywords: Exopolysaccharide, Lactic Acid Bakteria, Optimization, Secondary
Metabolite
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