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Aktivitas Biolarvasida Ekstrak Metanol Daun Jeruk Purut (Citrus Hystrix) Terhadap Larva Aedes Aegypti
Dengue Hemorrhagic Fever (DHF) is a significant public health problem transmitted by Aedes aegypti mosquitoes. One potential approach to control its transmission is by exploring the larvicidal activity of kaffir lime (Citrus hystrix) leaf extract as a safer and environmentally friendly biolarvicide. This study employed a true experimental design and was conducted from July 2024 to August 2025. The extract preparation was carried out at the Faculty of Mathematics and Natural Sciences Laboratory, Universitas Riau, while larval mortality testing was conducted at the Parasitology Laboratory, Faculty of Medicine, Universitas Riau. Larvae were collected from residential areas in Marpoyan Damai District. The extract was obtained through maceration using methanol as the solvent and tested at concentrations of 1%–5%, with 1% abate as the positive control and distilled water as the negative control. The experiment was performed in four replications. Larval mortality was observed at 1, 2, 3, 24, and 48 hours. Data analysis using one-way ANOVA demonstrated that Citrus hystrix leaf extract exhibited larvicidal activity against Aedes aegypti (p = 0.014 < 0.05). A concentration of 4% caused 94% larval mortality within 48 hours. Post Hoc LSD analysis revealed significant differences in larval mortality between concentrations of 1% and 4%, as well as 2% and 4%. These findings indicate that higher concentrations of Citrus hystrix leaf extract lead to increased larval mortality.
Keywords: Citrus hystrix leaf extract, Aedes aegypti larvae, biolarvicide, methanol, larval mortality.
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