CD Tesis
Dampak Kebakaran Lahan Gambut Terhadap Populasi Bakteri Selulolitik Pada Kebun Kelapa Sawit Dan Karet
Forest and agricultural land fires that occur almost every year in
Indonesia, especially Riau province. Fire that occurred in Riau Province. The
peatland fires that occurred in Riau Province in 2019 were quite extensive.
Ministry of Environment and Forestry data shows 75,871 ha of burned land in
Riau Province, divided into several districts and cities. Degradation of peatlands
can be caused by processing peat into agricultural land and it can also be caused
by fires of the peatlands themselves. One way to assess the extent of peat
degradation is by observing soil microorganisms. Soil microorganisms that play
an important role in decomposing organic matter into peat are cellulolytic
bacteria. Until now, there is very little research related to the degradation of
peatlands in kebun for oil palm and rubber.
Observation of cellulolytic bacteria can be done by counting the number
of bacterial colonies or by observing the enzyme activity of the cellulolytic
bacteria. From the observation data of cellulolytic bacteria on burned land and
then compared to unburned land, it can be assessed whether there is peat
degradation or not peat degradation on the burned land. After successfully
assessing peat degradation, we will get information that can be recommended to
the public and the authorities about peat damage in agricultural land due to fires.
This study aims to calculate the total population of cellulolytic bacteria and
determine the activity of cellulolytic enzymes qualitatively in each. microbial
isolates found and to determine the level of peatland degradation after fire.
The research was carried out by survey in the field, determining the
sample point using the purposive sampling method, which is choosing a location
according to the objectives of the research conducted at 4 locations. The first
location is oil palm land that is not burnt, the second location is oil palm land that
is burnt, the third location is unburned rubber land and the fourth location is
burned rubber land. Each location was repeated 3 times, in order to obtain 12
sample points for observation. Furthermore, at each sample point 2 soil depth
observations were made, namely the depth of 0-15 cm and 15-30 cm. so that 24
units of soil samples were obtained. Field observations include soil temperature,
air temperature, soil moisture, soil pH, and water level. Soil samples were
analyzed in the Pathology, Entomology and Microbiology laboratory at UIN
Suska Riau. Laboratory analysis includes total cellulotytic microbial analysis and
screening of cellulolytic enzyme activity qualitatively.
The results showed that the total population of cellulolytic bacteria in the
plantations area did not burn higher than the total population of cellulolytic
bacteria in the burned plantations area. The total number of cellulolytic bacteria
in unburned garden land ranged from 6.8 x 109 cfu / g to 13.9 109 cfu / g whereas
on burned garden land the total number of cellulolytic bacteria only ranged from
5.3 x 109 to 8.3 x 109 cfu / g. The total population of cellulolytic bacteria in
rubber plantations was higher than in oil palm plantations. The total population
xv
of bacteria in rubber plantation land ranged from 11.3 x 109 cfu / g to 13.9 x109
cfu / g compared to the total population of cellulolytic bacteria in oil palm
plantations which ranged from 6.8 x109 cfu / g to 9.9 x109 cfu / g. The total
population of cellulolytic bacteria at a soil depth of 0-15 cm is higher than the
soil depth of 15-30 cm. The total population of cellulolytic bacteria at a soil depth
of 0-15 cm ranged from 5.3 x 109 cfu / g to 13.9 x109 cfu / g higher than the total
population of cellulolytic bacteria at a soil depth of 15-30 cm ranging from 5.6
x109 cfu / g to 11.3 x 109 cfu / g. There are 8 cellulolytic bacterial isolates that
are able to degrade celluose. Cellulolytic enzyme activity from 4 locations and 2
depths was considered as high criteria, namely> 1.76. The highest cellulolytic
enzyme activity was produced by bacterial isolates from burnt rubber plantations
at a soil depth of 0-15 cm with a Z / K ratio of 5.25. From the results of the
assessment of the impact of fires in oil palm and rubber plantations, it was found
that there was land degradation in the plantation of oil palm and rubber after the
fire.
Keyword : Land Fire, Cellulolytic Bacteria, Rubber Plantation, Oil Palm
Plantation
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