Image of hubungan persentase metilasi dna dan ekspresi Mrna gen mesoderm spesific transcript (mest) oligozoospermia dan normozoospermia pada pasien infertilitas
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hubungan persentase metilasi dna dan ekspresi Mrna gen mesoderm spesific transcript (mest) oligozoospermia dan normozoospermia pada pasien infertilitas

Male infertility is a significant reproductive health issue often
associated with sperm quality. One approach to understanding its molecular
mechanism is by exploring epigenetic regulation, particularly DNA methylation
and gene expression. MEST is an imprinted gene essential for spermatogenesis
and has been implicated in infertility, especially in oligozoospermia.
Methods: This case control study included 48 spermatozoa samples from infertile
males, grouped into oligozoospermia and normozoospermia. DNA methylation
was analysed using MSP-PCR, and MEST mRNA expression was assessed by
qRT-PCR. The Mann-Whitney test was used to compare groups, and Spearman’s
rho was used for correlation analysis.
Results: DNA methylation percentage of the MEST gene were higher in the
oligozoospermia group, although the difference was not statistically significant (p
> 0.05). In contrast, mRNA expression of MEST was significantly higher in the
oligozoospermia group compared to normozoospermia (p < 0.05). A significant
negative correlation was found between DNA methylation and mRNA expression
in oligozoospermia (r = -0.513), while no significant correlation was observed in
normozoospermia.
Conclusion: The percentage of DNA methylation of the MEST gene did not differ
significantly between oligozoospermia and normozoospermia, but MEST mRNA
expression increased significantly in the oligozoospermia group. Furthermore, a
significant negative correlation was found between DNA methylation and MEST
mRNA expression in oligozoospermia, while in normozoospermia the correlation
was not significant.
Keywords: DNA methylation, mRNA expression, MEST gene, Oligozoospermia,
Normozoospermia

Ketersediaan
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Perpustakaan Universitas Riau 2110247734
2110247734
Tersedia
Informasi Detail
Judul Seri
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No. Panggil
2110247734
Penerbit
Pekanbaru : Universitas Riau – PASCA – Magister Ilmu Biomedis.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2110247734
Tipe Isi
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Tipe Media
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Tipe Pembawa
-
Edisi
-
Subjek
Magister Ilmu Biomedis
Info Detail Spesifik
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Pernyataan Tanggungjawab
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