CD Skripsi

pengaruh prakondisi vitamin d3 terhadap viabilitas, antioksidan dan apoptosis sel punca mesenkimal yang dipapar hidrogen peroksida

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Mesenchymal stem cells (MSCs) are well known for their significant therapeutic potential due to several key biological characteristics. However, MSCs have a limited lifespan during in vitro expansion. This limitation is primarily due to the accumulation of reactive oxygen species (ROS), which triggers oxidative stress and can potentially damage biomolecules within MSCs. The objective of this study was to investigate the effects of vitamin D3 preconditioning on the viability of MSCs exposed to hydrogen peroxide (H2O2), a known inducer of oxidative stress.
Methods: Mesenchymal stem cells were isolated from Wharton's jelly tissue of the umbilical cord, cultured until passage 3-5, then preconditioned with vitamin D3 (0-100 nM) for 24 hours, and exposed to H2O2 for 4 hours. Cell viability was evaluated using the cell counting kit-8 (CCK-8) assay, antioxidant levels were assessed through mRNA expression of SOD1, CAT, and GPx genes measured by qRT-PCR, and cell death was evaluated by examining the Annexin V/PI staining using flow cytometry.
Results: Exposure to H2O2 significantly reduced the viability of MSCs to 77.5% compared to those without H2O2 treatment. Preconditioning with vitamin D3 (0-100 nM) improved the viability of MSCs exposed to H2O2, raising it to 79.3-87.4%, making it comparable to the control group (without preconditioning). Additionally, the mRNA expression of antioxidant genes such as SOD1, CAT, and GPx in MSCs preconditioned with vitamin D3 showe no significant difference compared to the control group. Similarly, cell apoptosis and necrosis analysis via annexin V and PI staining yielded non-significant difference; the mean percentage of annexin V-stained MSCs in the vitamin D3 preconditioned group (73%) was relatively similar to the control group (68%), and the mean percentage of PI-stained MSCs in the vitamin D3 preconditioned group (0.3%) also did not differ significantly compared to the control group (0.5%).
Conclusion : Vitamin D3 preconditioning could enhance the viability of MSCs exposed to H2O2. However, vitamin D3 preconditioning did not affect antioxidant gene expression and cell death levels. These findings suggest that the increase in MSC viability due to vitamin D was not mediated through the regulation of antioxidant genes and cellular apoptosis.
Keywords: mesenchymal stem cells, vitamin D3, hydrogen peroxide, viability, apoptosis and antioxidant

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2110247770

Penerbit

Pekanbaru : Universitas Riau – PASCA – Magister Ilmu Biomedis., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2110247770

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Magister Ilmu Biomedis

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Pernyataan Tanggungjawab

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Versi lain/terkait

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