Hemorrhoids are anorectal disorders characterized by venous dilation and mucosal inflammation. Recent genomic analyses identify nitric oxide synthase-3 (NOS3) as a key risk gene; sapropterin—an FDA-approved 6-R-tetrahydrobiopterin analog for phenylketonuria—is predicted to down-regulate this pathway. Objective: To evaluate the anti-inflammatory effect of sapropterin on NOS3, cyclo-oxygenas…
Mesenchymal stem cells (MSCs) are well known for their significant therapeutic potential due to several key biological characteristics. However, MSCs have a limited lifespan during in vitro expansion. This limitation is primarily due to the accumulation of reactive oxygen species (ROS), which triggers oxidative stress and can potentially damage biomolecules within MSCs. The objective of this st…
Male infertility is a significant reproductive health issue often associated with sperm quality. One approach to understanding its molecular mechanism is by exploring epigenetic regulation, particularly DNA methylation and gene expression. MEST is an imprinted gene essential for spermatogenesis and has been implicated in infertility, especially in oligozoospermia. Methods: This case contro…
Oligozoospermia, occurs in 34.14% of male infertility cases, define as sperm concentration counted less than 15 million sperm/mL/ejaculation. One of the factor that affects sperm concentration is epigenetic factors, including DNA methylation. We hypotized that sperm concentration is affected by the DNA methylation of H19 gene, a significant gene in fetal growth and development, especially in th…