Dahlia tubers contain fungus Sporothrix sp. which have antimicrobial activity. The objective of this research was to determine the agitation (75,100,125,150 and 175 rpm) that effect the activity of antimicrobial in malt extract broth (MEB). The result showed that agitation of 150 rpm have the antimicrobial activity. It produced the inhibition zone of 21,00±0,58 mm againts Staphylococcus aureus…