CD Skripsi
Gambaran Profil Leukosit Ikan Jambal Siam (Pangasianodon Hypophthalmus) Yang Diberi Pakan Mengandung Larutan Daun Pepaya (Carica Papaya L.) Fermentasi
ABSTRACT
Papaya leaves (Carica papaya L.) contain tannins, alkaloids, flavonoids,
terpenoids, saponins, and karpain alkaloids which are antiseptic, anti-inflammatory,
antifungal, antibacterial and can be used as immunomodulators that can strengthen
the immune system. This study aimed to obtain the best dose of fermented papaya
leaf (Carica papaya L.) solution in increasing the non-specific immune response of
jambal siam fish by looking at total leukocytes, leukocyte differentiation, phagocytic
activity, and survival. The method used is an experimental method using a completely
randomized design (CRD) with five levels of treatment, namely (Kn): feeding
without being given a fermented papaya leaf solution and without being challenged
with A. hydrophila bacteria, (Kp): feeding without being given a leaf solution
fermented papaya and challenged with A. hydrophila bacteria, and feed added with
fermented papaya leaf solution with doses of P1 (16 ml/kg), P2 (18 ml/kg), P3 (20
ml/kg) and challenged with bacteria A. hydrophila. Fish were reared for 46 days. A.
hydrophila challenge test was performed on the 32nd day intramuscularly with a
density of 108 CFU/ml as much as 0.1 ml/head. The results showed that fermented
papaya leaf solution at a dose of 20 mL/kg of feed was the best dose to increase the
non-specific immune response of Jambal Siamese fish with an average total leukocyte
of 10.05 x104 cells/mm3, lymphocytes 82.00%, monocytes 4 ,67%, neutrophils
6.67%, platelets 6.67%, phagocytic activity 33.67%, and 96,67% survival.
Keywords: Leukocytes, Pangasianodon hypophthalamus, Fermentation, Carica
papaya L., Aeromonas hydrophila, lymphocytes
1. Student of the Faculty of Fisheries and Marine Affairs, University of Riau
2. Lecturer of the Faculty of Fisheries and Marine Affairs, University of Riau
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