CD Skripsi

Isolasi Senyawa Metabolit Sekunder Dan Uji Aktivitas Antibakteri Dari Ekstrak Etil Asetat Tumbuhan Paku Kawat (Lycopodiella Cernua (L.) Pic. Serm.)

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Lycopodiella cernua (L.) Pic. Serm. belongs to the family Lycopodiaceae. Members of this family have been traditionally used in various regions, particularly in China, India, and Southeast Asia, for the treatment of hepatitis, rheumatism, and burns. This plant is known to contain secondary metabolites, including steroids, phenolics, and flavonoids. The present study aims to isolate secondary metabolites from the ethyl acetate extract of L. cernua and to evaluate their antibacterial activity. Extraction was performed using maceration with a successive solvent system, namely n-hexane, dichloromethane (DCM), ethyl acetate, and methanol. The ethyl acetate extract was subjected to vacuum liquid chromatography (VLC), yielding four main fractions. Fraction 1 was further purified using flash column chromatography, resulting in 19 subfractions. Subfraction 10 underwent an additional round of flash chromatography, producing 53 vials. A yellow precipitate was observed in vial 51. Thin-layer chromatography (TLC) analysis of this vial indicated a single spot under UV light at 254 nm and 366 nm, as well as a positive reaction with anisaldehyde reagent. Purity testing of the compound showed a single spot on TLC using three different solvent systems, a melting point of 128–130 °C, and a single peak in the HPLC chromatogram with a retention time of 9.991 minutes. The isolated compound, designated as PK_EAF1_SF10_V51, was characterized using UV-Vis and FTIR spectroscopy, indicating that it belongs to the flavonoid group. This identification was further supported by LC-MS/MS data, PK_EAF1_SF110_V51 compound is suspected to be rutin, a well-known flavonoid. The antibacterial activity of the compound was evaluated against Bacillus cereus, Staphylococcus aureus, Salmonella typhimurium, and Vibrio parahaemolyticus. The minimum inhibitory concentration (MIC) values were 100 ppm for B. cereus and S. aureus, 125 ppm for V. parahaemolyticus, and 250 ppm for S. typhimurium. The minimum bactericidal concentration (MBC) values were found to be >200 ppm for all tested bacterial strains.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103126512

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103126512

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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