CD Skripsi
Uji Aktivitas Antibakteri Mikroba Endofit Paku Sarang Burung (Asplenium Nidus) Terhadap Bakteri Propionibacterium Acnes
Acne is one of the common skin diseases found in Indonesia. Acne or Acne vulgaris occurs due to infection or inflammation of the polysebaceous caused by blockage and accumulation of keratin material triggered by the presence of Propionibacterium acnes bacteria. Current acne treatment uses a lot of synthetic antibiotics, as a result if used for a long period of time it can increase resistance so that acne is difficult to cure. One effort to overcome drug resistance is to utilize endophytic bacteria that have the ability to produce similar compounds from their host plants including antibacterial compounds. Plants that have the potential as antibacterials are bird's nest ferns (Asplenium nidus). The content of this plant is flavonoids, alkaloids, terpenoids, phenolics, tannins, and saponins. This study aims to test the antibacterial activity of endophytic microbes isolated from the A. nidus plant against P. acnes bacteria. Antibacterial activity testing was carried out using the agar disk method. The isolation results obtained 16 endophytic microbes consisting of 11 bacterial isolates and 5 fungal isolates. Antibacterial activity test of endophytic microbes obtained 9 isolates of endophytic bacteria that were able to inhibit the growth of P. acnes with the highest antibacterial activity by BRAN 10 with an inhibition zone diameter of 13.47 mm. The highest endophytic fungal activity was obtained from isolate JRAN 5 with an inhibition zone diameter of 9.86 mm.
Key findings: Antibacterial activity, Asplenium nidus, endophytic microbes, Propionibacterium acnes, secondary metabolites.
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